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10 de septiembre de 2015
Mastitis: 15 pathogens + Staphylococcal B-lactamasa gene. Thermo Scientific Pathoproof ® Complete -16
10 de septiembre de 2015
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Mastitis: 11 pathogens + Staphylococcal B-lactamasa gene. Thermo Scientific Pathoproof ® Complete -12


The PathoProof Mastitis Complete-12 assay identifies 11 mastitis-causing microorganisms species or species groups and the β -lactamase penicillin resistance gene in staphylococci (including
Staphylococcus aureus and all major coagulase-negative staphylococci).

Código: PF0870S/L

Código: PF0870S/L. Categorías: , , , , , , .
   
Descripción

Description of the product

Thermo Scientific PathoProof Mastitis Complete assays are designed for accurate same-day identification of mastitis-causing microorganisms from bovine milk using quantitative real-time polymerase chain reaction (PCR).PCR technology is used for amplifying DNA in a test tube which then enables further analysis of DNA. In thePathoProof™ assays, real-time PCR detects the presence
of DNA in a milk sample and identifies the bacterial species in question based on its unique DNA. The kit
includes all the necessary reagents for bacterial DNA extraction and PCR. The test has been optimized for use with even the most challenging milk samples.
Real-time PCR has become the “gold standard” method for food pathogen testing and quality assurance. Based on this advanced technology, PathoProof Mastitis Complete assays offer several advantages over the conventional bacterial culture method:
  • Results are obtained substantially faster.
  • Risk of carry-over contamination in the laboratory is minimized because the tests are performed in closed reaction vessels that are not opened after the run.
  • Fewer “no growth” results because the test identifies and quantifies DNA, so it accurately detects viable, dead and growth-inhibited microorganisms.
  • Applicable for use with preserved milk samples, thus eliminating the need for cooling during sample transportation.
  • Can be integrated into milk recording programs using preserved milk.
The small reagent kits are sufficient for 50 tests*, and the large kits for 4×96 tests**. The kits are stable for six months from the packaging date when stored and handled properly.
The PathoProof Mastitis Complete-12 assay identifies 11 mastitis-causing microorganisms species or species groups and the β-lactamase penicillin resistance gene in staphylococci (including Staphylococcus aureus and all major coagulase-negative staphylococci). The bacteria and the β-lactamase gene are detected in four separate PCR reactions. The PathoProof Mastitis Complete-12 assay can identify the following bacterial species and groups:
  • Staphylococcus aureus
  • Enterococcus spp.(including E. faecalis and E. faecium)
  • Corynebacterium bovis
  • Staphylococcal β-lactamase gene (the gene responsible for penicillin resistance in staphylococci)
  • Escherichia coli
  • Streptococcus dysgalactiae
  • Staphylococcus spp. (including Staphylococcus aureus and all relevant coagulase-negative staphylococci)
  • Streptococcus agalactiae
  • Streptococcus uberis
  • Klebsiella oxytoca and/or K. pneumoniae
  • Serratia marcescens
  • Arcanobacterium pyogenes and/or Peptoniphilus indolicus

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Reacciones

50 rxns, 4×96 rxns

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