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Bacillus cereus Real Time PCR Kit *CE Marked


DD-0042

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Descripción

1. Intended Use
The Bacillus Cereus real time PCR Kit is a test for the detection of Bacillus Cereus in stool or vomit
samples in real time PCR systems.

2. Product Description
Bacillus cereus is an endemic, soil-dwelling, Gram-positive, rod-shaped, beta hemolytic bacteria that
causes foodborne illness. It is the cause of “Fried Rice Syndrome”. B. cereus bacteria are facultative
anaerobes, and like other members of the genus Bacillus can produce protective endospores. B. cereus
is responsible for a minority of foodborne illnesses (2–5%). It is known to create heavy nausea,
vomiting, and abdominal periods. Generally speaking, Bacillus foodborne illnesses occur due to
survival of the bacterial spores when food is improperly cooked. This problem is compounded when
food is then improperly refrigerated, allowing the spores to germinate. Bacterial growth results in
production of enterotoxin, and ingestion leads to two types of illness, diarrheal and emetic syndrome.
The Bacillus Cereus real time PCR Kit contains a specific ready-to-use system for the detection of the
Bacillus Cereus using PCR (polymerase chain reaction) in the real-time PCR system. The master
contains reagents and enzymes for the specific amplification of the Bacillus Cereus DNA.
Fluorescence is emitted and measured by the real time systems´ optical unit during the PCR. The
detection of amplified Bacillus Cereus DNA fragment is performed by ROB reaction mix in
fluorimeter channel FAM with the fluorescent quencher BHQ1. Toxin types are identified by
HBL&NHE reaction mix. In addition, the kit contains a system to identify possible PCR inhibition by
measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control
defined as 1×107 copies/ml is supplied which allow the determination of the gene load. For further
information, please refer to section 9.3 Quantitation.

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