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Bordetella pertussis
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Bordetella Pertussis Real Time PCR Kit *CE Marked


RD-0061

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Descripción

1. Intended Use
Bordetella Pertussis real time PCR Kit is used for the detection of bordetella pertussis by real time PCR
systems in samples like nasal and pharyngeal secretions, sputum, and etc.

2. Product Description
Bordetella pertussis is the cause of one of the most contagious human diseases known as whooping
cough. B. pertussis causes severe coughing spells, with a characteristic “whoop” made as the affected
person struggles to breathe through narrowed airway passages between coughing spasms. B. pertussis
is a small gram-negative aerobic coccobacillus that colonizes the cilia of the nose and throat of infected
humans. Toxins produced by B. pertussis paralyze the cilia and cause inflammation of the respiratory
tract, interfering with the clearance of pulmonary secretions. This disease was first described in the 16th
century and was one of the most frequent and severe diseases in infants in the United States, commonly
resulting in morbidity and mortality among children prior to introduction of an effective vaccine. The
incidence decreased dramatically following the introduction of the vaccine; however, incidence has
been gradually increasing since the early 1980’s. One explanation for the increase might be the
adaptation of B. pertussis bacteria to vaccine-induced immunity.Bordetella pertussis real time PCR kit
contains a specific ready-to-use system for the detection of Bordetella Pertussis by polymerase chain
reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific
amplification of the bordetella pertussis DNA. Fluorescence is emitted and measured by the real time
systems´ optical unit during PCR. The detection of amplified bordetella pertussis DNA fragment is
performed in fluorimeter channel FAM with the fluorescent quencher BHQ1. DNA extraction buffer is
available in the kit. In addition, the kit contains a system to identify possible PCR inhibition by
measuring the HEX/VIC/JOE fluorescence of the internal control (IC).An external positive
control(1×107copies/ml) contained, allow the determination of the gene load. For further information,
please refer to section 9.3 Quantitation.

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