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Botulinus (Types A/B/E/F) Real Time PCR Kit


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1. Intended Use
Botulinus real time PCR kit is used for the detection of Botulinus (Types A/B/E/F)in stool ,water and
food samples by using real time PCR systems.

2. Product Description
Botulinum (or Clostridium botulinum)is the aetiological agent of botulism, a disease marked by
flaccid paralysis that can progress to asphyxiation and death. This species is defined by the production
of one of the botulinum neurotoxins (BoNTs), which are the most potent toxins known. Botulinum has
been classified as a category A select agent. There are four related but antigenically distinct BoNT
types that cause disease in humans, A, B, E and F.
Botulinus real time PCR kit contains a specific ready-to-use system for the detection of the Botulinus
through polymerase chain reaction in the real-time PCR system. The master contains reagents and
enzymes for the specific amplification of the Botulinus DNA. Fluorescence is emitted and measured
by the real time systems´ optical unit during the PCR. The detection of BoNT type A and B is
performed in one Reaction Mix and BoNT type E and F is performed in another one with the
fluorescent quencher BHQ1.An external positive control defined as 1×107copies/ml is supplied which
allow the determination of the gene load. For further information, please refer to section 9.2

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