1. Intended Use
C.jejuni real time PCR Kit is used for the detection of C.jejuni in stool or water samples by
LightCycler (Roche) real time PCR systems.
2. Product Description
Campylobacter jejuni is a Gram-negative slender, curved, and motile rod. It is the leading cause of
food poisoning, being three times more common than Salmonella. This bacterium is now recognized
as an important enteric pathogen. Usually C.jejuni outbreaks are small (less than 50 people). C.jejuni
infection causes diarrhea, which may be watery or sticky and can contain blood and fecal leukocytes.
Other symptoms often present are fever, abdominal pain, nausea，headache and muscle pain.
C.jejuni real time PCR kit contains a specific ready-to-use system for the detection of C.jejuni by
polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and
enzymes for the specific amplification of C.jejuni DNA. Fluorescence is emitted and measured by
the real time systems´ optical unit during PCR. The detection of amplified C.jejuni DNA fragment is
performed in fluorimeter channel FAM with the fluorescent quencher BHQ1. DNA extraction
buffer is available in the kit. In addition, the kit contains a system to identify possible PCR inhibition
by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive
control (1×108copies/ml) contained, allows the determination of the gene load. For further
information, please refer to section 9.3 Quantitation.