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Chlamydia trachomatis
14 de septiembre de 2015
CT&NG RealTime PCR Kit
14 de septiembre de 2015
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Chlamydia Trachomatis(CT)Real Time PCR Kit


SD-0016

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Descripción

1. Intended Use
Chlamydia trachomatis real time PCR kit is used for the detection of Chlamydia trachomatis in genital
swabs samples by using real time PCR systems.

2. Product Description
Chlamydia trachomatis is a small bacterium that cannot grow outside a living cell. In this respect it
resembles a virus, but it is actually a very sophisticated organism. It is a natural pathogen to humans.
Worldwide, the most important disease caused by Chlamydia trachomatis is trachoma, one of the
commonest infectious causes of blindness. In some parts of the developing world, over 90% of the
population becomes infected. The organism often causes genital tract infection. In men, Chlamydia
trachomatis is the commonest cause of non-gonococcal or non-specific urethritis. In women, the
organism may infect both the cervix and the urethra. Epididymitis may complicate the infection in men,
whilst in women infection in the upper genital tract, may lead to acute pelvic inflammatory disease
(PID).Chlamydia trachomatis real time PCR kit contains a specific ready-to-use system for the detection
of the Chlamydia trachomatis by polymerase chain reaction in the real-time PCR system. The master
contains reagents and enzymes for the specific amplification of the chlamydia trachomatis DNA.
Fluorescence is emitted and measured by the real time systems´ optical unit during PCR. The detection
of amplified chlamydia trachomatis DNA fragment is performed in fluorimeter channel 530nm with the
fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and genital swabs samples are
used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR
inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control
(1×107copies/ml) contained, allow the determination of the gene load.

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