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Chlamydia Trachomatis(CT)Real Time PCR Kit
14 de septiembre de 2015
Haemophilus ducreyi DNA
14 de septiembre de 2015
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CT&NG RealTime PCR Kit


SD-0025-A

Categorías: , , , , .
   
Descripción

1. Intended Use
CT&NG real time PCR kit is used for the detection of Chlamydia trachomatis and Neisseria
Gonorrhoeae in genital swabs samples by using real time PCR systems.

2. Product Description
Neisseria gonorrhoeae and Chlamydia trachomatis are recognized as two of the most prevalent
sexually transmitted bacterial infections. Worldwide, there is an estimated annual incidence of 25
million cases of gonorrhea and 50 million cases of chlamydia. In an effort to prevent the spread of
these diseases, increased attention is being focused on early diagnosis and treatment of
symptomatic or asymptomatic infected individuals. In men, C. trachomatis causes 40 to 50% of
cases of nongonococcal urethritis, making it one of the most common sexually transmitted diseases
(STD) in heterosexual males. In women, chlamydia is a major cause of pelvic inflammatory
disease leading to infertility and ectopic pregnancy and can result in conjunctivitis and pneumonia
in newborn infants exposed during passage through an infected birth canal.Symptoms and
complications of gonorrhea are similar to those of chlamydia, and a substantial proportion of
infected individuals,especially women, are asymptomatic. For both organisms,asymptomatic
persons serve as a reservoir of infection,and since coinfection is common, symptoms may overlap,
making clinical diagnosis difficult.
CT&NG real time PCR kit contains a specific ready-to-use system for the detection of the CT&NG
by polymerase chain reaction in the real-time PCR system. The master contains reagents and
enzymes for the specific amplification of the chlamydia trachomatis DNA. Fluorescence is emitted
and measured by the real time systems´ optical unit during PCR. The detection of amplified
Chlamydia trachomatis DNA fragment is performed in fluorimeter channel FAM with the
fluorescent quencher BHQ1. Detection of amplified Neisseria gonorrhoeae DNA fragment is
performed in fluorimeter channel HEX/VIC/JOE with the fluorescent quencher BHQ1. DNA
extraction buffer is available in the kit and genital swabs samples are used for the extraction of the
DNA. An external positive control (1×107copies/ml) contained, allow the determination of the gene
load.

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