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Enteric Adenovirus Real Time PCR Kit *CE Marked


DD-0043

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Descripción

1. Intended Use
Enteric Adenovirus real time PCR kit is used for the detection of Adenovirus in stool, sputum, gargle,
or blood samples by real time PCR systems.

2. Product Description
Human adenoviruses are classified into 47 serotypes and six subgenera (A-F) with different tropisms.
In recent years adenovirus type 40 (Ad40) and 41 (Ad41) of subgenus F have been shown to be
causative agents in enteric infections, which is second in importance only to rotaviruses as a cause of
infantile gastroenteritis. Infection with EAds occurs worldwide and has been associated with 4-17% of
cases of diarrhoea in children. AD40 and Ad41 primarily affect young children less than 2 years of age
and occur throughout the year.
Enteric Adenovirus real time PCR kit contains a specific ready-to-use system for the detection of
enteric adenovirus by polymerase chain reaction (PCR) in the real-time PCR system. The master
contains reagents and enzymes for the specific amplification of the Adenovirus DNA including type
1,2 and 5.. Fluorescence is emitted and measured by the real time systems´ optical unit during PCR.
The detection of amplified Adenovirus DNA fragment is performed in fluorimeter channel FAM with
the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and stool, sputum, gargle,
nasopharyngeal swab or blood samples are used for DNA extraction. In addition, the kit contains a
system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the
internal control (IC). An external positive control (1×107 copies/ml) contained, allows the
determination of the gene load. For further information, please refer to section 9.3 Quantitation.

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