Hantavirus pulmonary syndrome real time RT-PCR kit is used for the detection of Hantavirus pulmonary
syndrome (HPS) in serum by using real time PCR systems.
Hantavirus pulmonary syndrome real time RT-PCR kit contains a specific ready-to-use system for the
detection of Hantavirus pulmonary syndrome by Reverse Transcription Polymerase Chain Reaction
(RT-PCR) in the real-time PCR system. The master contains Super Mix for the specific amplification of
hanta virus RNA. The reaction is done in one step real time RT-PCR. The first step is a reverse
transcription (RT), during which the hanta virus RNA is transcribed into cDNA. Then, a thermostable
DNA polymerase is used to amplify the specific gene fragments by polymerase chain reaction.
Fluorescence is emitted and measured by the real time systems´ optical unit. The detection of amplified
Hanta virus (HPS) DNA fragment is performed in fluorimeter channel FAM with the fluorescent
quencher BHQ1. In addition, the kit contains a system to identify possible PCR inhibition by measuring
the VIC/JOE fluorescence of the internal control (IC). An external positive control (1×10
7copies/ml) contained, allows the determination of the gene load. For further information, please refer to section 10.3 Quantitation.