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HBV Quantitative Real Time PCR Kit


HD-0002

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Descripción

1. Intended Use
HBV quantitative real time PCR kit is used for the detection of HBV in serum or plasma by using real time PCR systems.
Its characteristics:
High sensitivity: lower detection line 500 IU/ml;LOQ:103 ~108 IU/ml
High specificity: test result will be positive, only to hepatitis B virus.
Short operating time: One and a half hours totally
Good stability: kept for 12 months at –20℃; CV≤5%;

2. Product Description
Chronic hepatitis B virus (HBV) infection affects over 350 million people worldwide and over 1 million die annually of HBV-related chronic liver
disease. Although many individuals eventually achieve a state of nonreplicative infection, the prolonged immunologic response to infection may lead to
the development of cirrhosis, liver failure, or hepatocellular carcinoma (HCC) in up to 40% of patients. HBV is an enveloped virus in the family
Hepadnaviridae, with partially double-stranded DNA and 42nm in diameter. Its antigenic components includes hepatitis B surface antigen (HBsAg),
hepatitis B core antigen (HBcAg), and hepatitis B e antigen (HBeAg). Virus replicates in liver and sheds into blood in high concentration. HBV is
transmitted through blood and body fluid.
HBV real time PCR kit contains a specific ready-to-use system for HBV detection (for genotype A~H) through polymerase chain reaction (PCR) in the
real-time PCR system. The master contains reagents and enzymes for the specific HBV DNA amplification. Fluorescence is emitted and measured by the
real time systems´ optical unit during PCR. The detection of amplified HBV DNA fragment is performed in fluorimeter channel FAM with the
fluorescent quencher BHQ1. DNA extraction buffer is available in the kit. In addition, the kit contains a system to identify possible PCR inhibition by
measuring the HEX/VIC/JOE fluorescence of the internal control (IC). Four quantitation standards are supplied, allows the determination of the gene
load.

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