HBV Quantitative Real Time PCR Kit
10 de septiembre de 2015
HCV Real Time RT-PCR Kit
10 de septiembre de 2015
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HCV Genotype Real Time RT-PCR Kit


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1. Intended Use
HCV genotype real time RT-PCR kit is used for the detection of HCV genotype 1 in blood serum by
using real time PCR systems.

2. Product Description
Hepatitis C virus has at least six forms or genotypes. HCV genotypes and subtypes are distributed
variously in different parts of the world. Genotypes 1-3 are widely distributed throughout the world.
Subtype 1a is prevalent in North and South America, Europe, and Australia. Subtype 1b is common in
North America and Europe, and is also found in parts of Asia. Genotype 2 exists in most developed
countries, and is less common than genotype 1. Some studies suggest that different types of HCV may
be related to different transmission routes. HCV genotype 1 is significantly associated with human
immunodeficiency virus. Genotype 1 is related to a poor response to treatment. Genotyping can help
doctor determine an appropriate hepatitis C treatment and how long treatment should be given.
HCV genotype real time RT-PCR kit contains a specific ready-to-use system for the detection of HCV
genotype 1 by Reverse Transcription Polymerase Chain Reaction (RT-PCR) in the real-time PCR
system. The master contains Super Mix for the specific amplification of HCV RNA and HCV
genotype1 RNA. Super Mix A is specific for HCV RNA; Super Mix B is specific for HCV genotype1
RNA. The reaction is done in one step real time RT-PCR. The first step is a reverse transcription (RT):
HCV RNA is transcribed into cDNA. Then, a thermostable DNA polymerase is used to amplify the
specific gene fragments by polymerase chain reaction (PCR).Fluorescence is emitted and measured by
the real time systems´ optical unit during PCR. The detection of amplified HCV DNA fragment is
performed in fluorimeter channel FAM with the fluorescent quencher BHQ1.

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