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HEV Ⅳ Real Time RT-PCR Kit *CE Marked
10 de septiembre de 2015
Low-concentration Hepatitis B Virus Real Time PCR Kit
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HGV Real Time RT-PCR Kit *CE Marked


HR-0012

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Descripción

1. Intended Use
HGV real time RT-PCR kit is used for the detection of HGV in serum or plasma sample by using real
time PCR systems.

2. Product Description

Hepatitis G virus, also called GB-C virus, was initially thought to be associated with chronic hepatitis,
extensive investigation failed to identify any association between this virus and any clinical illness.
The precise role of HGV/GB-C in human disease is currently under investigation.
Phylogenetically closely related to hepatitis C virus(85% similarity), Hepatitis G appears to replicate
primarily in lymphocytes, and poorly if at all in hepatocytes.
Approximately 2% of healthy US blood donors are viraemic with HGV, and up to 13% of blood
donors have antibodies to E2 protein, indicating prior infection.
Parenteral, sexual and vertical transmission of HGV have all been documented, and because of shared
modes of transmission, individuals infected with HIV are commonly co-infected with HGV. Among
people with HIV infection, the prevalence of HGV viraemia ranges from 14 to 43%.
HGV real time RT-PCR kit contains a specific ready-to-use system for HGV detection by Reverse
Transcription Polymerase Chain Reaction (RT-PCR) in the real-time PCR system. The master contains
Super Mix for the specific amplification of HGV RNA. The reaction is done in one step real time
RT-PCR. The first step is a reverse transcription (RT): HGV RNA is transcribed into cDNA. Then, a
thermostable DNA polymerase is used to amplify the specific gene fragments by polymerase chain
reaction. Fluorescence is emitted and measured by the real time systems´ optical unit. The detection of
amplified HGV DNA fragment is performed in fluorimeter channel FAM with the fluorescent
quencher BHQ1. In addition, the kit can be used for identification of possible PCR inhibition by
measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control
defined as 1×10 7
copies/ml is supplied to allow the determination of the gene load. For further
information, please refer to section 9.3 Quantitation.

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