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HIV-2 Real Time RT-PCR Kit


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1. Intended Use
HIV-2 real time RT-PCR kit is used for the detection of HIV-2 in serum by using real time PCR

2. Product Description
Human immunodeficiency virus (HIV) is a retrovirus that can lead to acquired immunodeficiency
syndrome (AIDS). Infection with HIV occurs by the transfer of blood, semen, vaginal fluid,
pre-ejaculate, or breast milk. HIV infection in humans is now pandemic. Two species of HIV infect
humans: HIV-1 and HIV-2. HIV-1 is more virulent. It is easily transmitted and is the cause of the
majority of HIV infections globally. HIV-2 is less transmittable and is largely confined to West
HIV-2 real time RT-PCR kit contains a specific ready-to-use system for HIV-2 detection through
Reverse Transcription Polymerase Chain Reaction (RT-PCR) in the real-time PCR system. The
master contains Super Mix for the specific amplification of HIV-2 RNA including subtype A~G.
The reaction is done in one step real time RT-PCR. The first step is a reverse transcription (RT),
during which the HIV-2 RNA is transcribed into cDNA. Then, a thermostable DNA polymerase is
used to amplify the specific gene fragments by polymerase chain reaction (PCR). Fluorescence is
emitted and measured by the real time systems´ optical unit during the PCR. The detection of
amplified HIV-2 DNA fragment is performed in fluorimeter channel 530nm with the fluorescent
quencher BHQ1. In addition, the kit can be used for identification of possible PCR inhibition by
measuring the 560nm fluorescence of the internal control (IC). An external positive control defined
as 1×107IU/ml is supplied. It allows the determination of the gene load. For further information,
please refer to section 9.3 Quantitation.

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