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HSVⅠ&Ⅱ Real Time PCR Kit *CE Marked


SD-0017

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Descripción

1. Intended Use
HSV Ⅰ&Ⅱreal time PCR kit is used for the detection of HSV Ⅰ&Ⅱ in serum, herpes secretion or
genital swabs samples by using real time PCR systems.

2. Product Description

Herpes simplex virus (HSV) is one of the most common agents infecting humans of all ages. The virus
occurs worldwide and produces a variety of illnesses, including mucocutaneous infections, infections of
the CNS, and occasionally infections of the visceral organs. It is also a sexually transmitted disease (STD)
caused by the herpes simplex viruses typeⅠ(HSV-Ⅰ)and typeⅡ(HSV-Ⅱ). HSVⅠ&Ⅱreal time PCR kit
contains a specific ready-to-use system for the detection of the HSVⅠ&Ⅱby polymerase chain reaction
in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of
the HSVⅠ&ⅡDNA. Fluorescence is emitted and measured by the real time systems´ optical unit during
PCR. The detection of amplified HSVⅠ&ⅡDNA fragment is performed in fluorimeter channel FAM
with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum, herpes
secretion and genital swabs samples are used for DNA extraction. In addition, the kit contains a system to
identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC).
An external positive control (1×107copies/ml) contained, allows the determination of the gene load. For
further information, please refer to section 9.3 Quantitation.

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