MP real time PCR kit is used for the detection of mycoplasma pneumoniae by real time PCR systems in samples like nasal and pharyngeal secretions and swabs, sputum, provoked sputum, bronchial lavage, lung biopsy，pleural effusion and etc.
2. Product Description
Mycoplasma pneumoniae is an important etiological agent of community acquired infections of upper and lower respiratory tracts in children and young adults, mainly atypical pneumonia. M. pneumoniae has been reported as the cause of 25-30 % of all pneumonia cases. This pathogen has also been associated with non respiratory diseases as meningitis, encephalitis, pancreatitis or arthritis.
Mycoplasma Pneumoniae real time PCR kit contains a specific ready-to-use system for the detection of mycoplasma pneumoniae by polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of MP DNA. Fluorescence is emitted and measured by the real time systems´ optical unit during PCR. The detection of amplified MP DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC).