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Norovirus Genogroup II Real Time RT-PCR Kit


DR-0231

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Descripción

1. Intended Use
Norovirus genogroupⅡreal time RT-PCR kit is used for the detection of norovirus genogroupⅡ in
stool or diarrhea samples by using real time PCR systems.

2. Product Description

Norovirus (NV), one genus of the family of Caliciviridae, is one of the most commonly reported
etiological agents of non-bacterial gastroenteritis in human world-wide. Hospitals, residential
facilities/nursing homes and schools/nurseries were most frequently affected. To prevent further
spreading of the causative agent during a mass outbreak especially in semi closed communities, such
as hospitals and nursing homes, an immediate application of hygiene measures as well as rapid and
sensitive diagnostics are needed. The increasing knowledge of the molecular properties of caliciviruses
led to the development of different assays for the detection of norovirus RNA and viral antigen. Since
enzyme immunoassays were found to be insufficient sensitive and/or insufficient specific so far
RT-PCR assays have become the methods of choice. Due to the high sequence diversity of human NV
which is classified into 3 genogroups (GI, GII, and GIV) containing at least 7 different genotypes in GI
(ie,.Norwalk virus) and 12 genotypes in GII. The genogroup specific oligonucleotide probes are used
in this real-time PCR Kit.
The norovirus genogroup Ⅱ real time RT-PCR kit contains a specific ready-to-use system for the
detection of the norovirus Group Ⅱusing RT-PCR (Reverse Transcription Polymerase Chain Reaction)
in the real-time PCR system. The master contains a Super Mix for the specific amplification of the
norovirus RNA. The reaction is done in one step real time RT-PCR. The first step is a reverse
transcription (RT), during which the norovirus genogroup ⅡRNA is transcribed into cDNA.
Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by means
of PCR (polymerase chain reaction). Fluorescence is emitted and measured by the real time systems´
optical unit during the PCR. The detection of amplified norovirus genogroupⅡRNA fragment is
performed in fluorimeter channel FAM with the fluorescent quencher BHQ1. In addition, the kit
contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of
the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which
allow the determination of the gene load. For further information, please refer to section 9.3
Quantitation.

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