1. Intended Use
By using real time PCR systems, Parechovirus real time PCR kit is used for the detection of
Parechovirus in samples like nasal and pharyngeal secretions, sputum, provoked sputum, stool,
C.S.F, serum (non-heparin anticoagulant) and etc.
2. Product Description
Parechovirus is a viral genus in the family Picornaviridae. The genus is composed of two species:
Human parechovirus and Ljungan virus.Six types of human parechovirus have been identified:
human parechovirus 1 (formerly echovirus 22), human parechovirus 2 (formerly echovirus 23), and
human parechoviruses 3, 4, 5 and 6, respectively. Human parechoviruses cause mild,
gastrointestinal or respiratory illness, but have been implicated in cases of myocarditis and
encephalitis. Human parechoviruses are commonly spread and more than 95% of humans are
infected by human parechoviruses early in life, within two to five years of age. The Ljungan virus
was first isolated from bank voles (Myodes glareolus, formerly Clethrionomys glareolus).Ljungan
virus has been proposed as a zoonotic virus, associated with diabetes and intrauterine fetal death in
human. However, the data regarding these features is currently limited and needs to be confirmed.
The reaction is done in one step real time RT-PCR. The first step is a reverse transcription (RT),
during which the Parechovirus RNA is transcribed into cDNA. Afterwards, a thermostable DNA
polymerase is used to amplify the specific gene fragments by means of PCR (polymerase chain
reaction). Fluorescence is emitted and measured by the real time systems´ optical unit during the
PCR. The detection of amplified Parechovirus DNA fragment is performed in fluorimeter channel
FAM with the fluorescent quencher BHQ1. In addition, the kit contains a system to identify
possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC).
An external positive control defined as 1×107copies/ml is supplied which allow the determination
of the gene load. For further information, please refer to section 9.3 Quantitation.