1. Intended Use
Plesiomonas shigelloides real time PCR kit is used for the detection of Plesiomonas shigelloides in
stool, vomit or water samples by using real time PCR systems.
2. Product Description
Plesiomonas shigelloides is a species of bacteria. It is a Gram-negative, rod-shaped bacterium which
has been isolated from freshwater, freshwater fish, and shellfish and from many types of animals
including cattle, goats, swine, cats, dogs, monkeys, vultures, snakes, and toads.Infections from this
organism cause gastroenteritis, followed by septicemia in immune deficient patients. It is placed
among the Enterobacteriaceae.
Plesiomonas shigelloides real time PCR kit contains a specific ready-to-use system for the detection of
the Plesiomonas shigelloides through polymerase chain reaction in the real-time PCR system. The
master contains reagents and enzymes for the specific amplification of the Plesiomonas shigelloides
DNA. Fluorescence is emitted and measured by the real time systems´ optical unit during the PCR.
The detection of amplified Plesiomonas shigelloides DNA fragment is performed in fluorimeter
channel FAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit. In
addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE
fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is
supplied which allow the determination of the gene load. For further information, please refer to