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11 de septiembre de 2015
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11 de septiembre de 2015
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Respiratory Adenovirus Real Time PCR Kit *CE Marked


RD-0195

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Descripción

1. Intended Use
Respiratory Adenovirus real time PCR kit is used for the detection of Adenovirus in sputum, gargle,
nasopharyngeal swab or blood samples by real time PCR systems.

2. Product Description
Human adenoviruses are classified into 47 serotypes and six subgenera (A-F) with different tropisms.
Under most circumstances, the clinically important Ad species are those associated with febrile
respiratory disease (AdB1, AdC, and AdE) since these can cause widespread outbreaks with severe
clinical presentations, including viral pneumonia and death.
Respiratory Adenovirus real time PCR kit contains a specific ready-to-use system for the detection of
respiratory adenovirus by polymerase chain reaction (PCR) in the real-time PCR system. The master
contains reagents and enzymes for the specific amplification of the Adenovirus DNA including type
1,2 and 5.. Fluorescence is emitted and measured by the real time systems´ optical unit during PCR.
The detection of amplified Adenovirus DNA fragment is performed in fluorimeter channel FAM with
the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and stool, sputum, gargle,
nasopharyngeal swab or blood samples are used for DNA extraction. In addition, the kit contains a
system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the
internal control (IC). An external positive control (1×107copies/ml) contained, allows the determination
of the gene load. For further information, please refer to section 9.3 Quantitation.

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