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Salmonella & Shigella Real Time PCR Kit *CE Marked
11 de septiembre de 2015
Salmonella spp. (all pathogenic)
11 de septiembre de 2015
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Salmonella paratyphi Real Time PCR Kit *CE Marked


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Descripción

1. Intended Use
Salmonella paratyphi real time PCR kit is used for the detection of Salmonella paratyphi in stool,
water or blood samples by using real time PCR systems.

2. Product Description
Salmonella paratyphi is part of the Enterobacteriaceae family; it is a Gram-negative motile, aerobic
rod which is facultatively anaerobic and there is serological identification of somatic and flagellar
antigens. Salmonella paratyphi causes bacterial enteric fever which is characterised by an abrupt onset,
continued fever, malaise, headache, anorexia, enlargement of spleen, bradycardia, rose spots on trunk
occur on approximately 25% of Caucasians, constipation is more common than diarrhea in adults;
complications include perforation/hemorrhage/ulceration of the intestines, less frequently psychosis,
hepatitis, cholecystitis, pneumonitis, and pericarditis. Salmonella paratyphi occurs sporadically or in
limited outbreaks and is restricted to humans. It is transmitted by direct or indirect contact with faeces
or rarely the urine of a patient or carrier, contaminated food, especially milk, milk products and
shellfish, it may be contaminated by the hands of a carrier or flies may be a possible vector.
Salmonella paratyphi real time PCR kit contains a specific ready-to-use system for the detection of the
Salmonella paratyphi using PCR (polymerase chain reaction) in the real-time PCR system. The master
contains reagents and enzyme for the specific amplification of the Salmonella paratyphi DNA.
Fluorescence is emitted and measured by the real time systems´ optical unit during the PCR. The
detection of amplified Salmonella paratyphi DNA fragment is performed in fluorimeter channel FAM
with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit. An external
positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.
For further information, please refer to section 9.2 Quantitation.

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