1. Intended Use
Salmonella & Shigella Real Time PCR Kit is used for the detection of Salmonella & Shigella in stool
or water samples by real time PCR systems.
2. Product Description
Salmonella is a genus of bacteria that are a major cause of foodborne illness throughout the world. The
bacteria are generally transmitted to humans through consumption of contaminated food of animal
origin, mainly meat, poultry, eggs and milk.
The symptoms of Salmonella infection usually appear 12–72 hours after infection, and include fever,
abdominal pain, diarrhoea, nausea and sometimes vomiting. The illness usually lasts 4–7 days, and
most people recover without treatment. However, in the very young and the elderly, and in cases when
the bacteria enter the bloodstream, antibiotherapy may be needed.
Shigella, its enterotoxicity can make the disease clinically appear as a diarrhea. The organism invades
the epithelial lining layer but does not penetrate. Usually within 2-3 days, dysentery results from
bacteria damaging the epithelial layers lining the intestine, often with release of mucus and blood and
attraction of leukocytes. However, watery diarrhea is frequently observed with no evidence of
dysentery. Shigella is primarily a disease of young children occurring by fecal-oral contact. Adults can
catch this disease from children, although it can be transmitted by infected adult food handlers who
contaminate food. The source in each case is unwashed hands. Man is the only “reservoir”.
Salmonella & Shigella real time PCR kit contains a specific ready-to-use system for the detection of
the Salmonella & Shigella by Polymerase Chain Reaction in the real-time PCR system. The master
contains reagents and enzymes for the specific amplification of Salmonella & Shigella DNA.
Fluorescence is emitted and measured by the real time systems´ optical unit during PCR. The detection
of amplified Salmonella DNA fragment is performed in fluorimeter channel HEX/VIC/JOE with the
fluorescent quencher BHQ1. The detection of amplified Shigella DNA fragment is performed in
fluorimeter channel FAM with the fluorescent quencher BHQ1.For further information, please refer to
section 9.2 Quantitation.