1. Intended Use
Salmonella Typhi real time PCR kit is used for the detection of Salmonella Typhi in stool, blood or
water samples by using real time PCR systems.
2. Product Description
Typhoid fever is caused by typhoid bacillus. It is characterized by the sudden onset of sustained fever,
severe headache, nausea, loss of appetite, constipation or sometimes diarrhoea. Severe forms have
been described with mental dullness and meningitis. Case-fatality rates of 10% can be reduced to less
than 1% with appropriate antibiotic therapy. However, strains resistant to chloramphenicol and other
recommended antibiotics (ampicillin, cotrimoxazole and even ciprofloxacin) have become prevalent in
several areas of the world. Paratyphoid fever can be caused by any of three serotypes of S. paratyphi A,
B and C. It is similar in its symptoms to typhoid fever, but tends to be milder, with a lower fatality rate.
Salmonella Typhi real time PCR kit contains a specific ready-to-use system for the detection of the
Salmonella Typhi by polymerase chain reaction in the real-time PCR system. The master contains
reagents and enzymes for the specific amplification of the Salmonella Typhi DNA. Fluorescence is
emitted and measured by the real time systems´ optical unit. The detection of amplified Salmonella
Typhi DNA fragment is performed in fluorimeter channel FAM with the fluorescent quencher BHQ1.
DNA extraction buffer is available in the kit. An external positive control (1×107copies/ml) contained,
allows the determination of the gene load. For further information, please refer to section 9.2