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Staphylococcus aureus
11 de septiembre de 2015
Vancomycin-resistant Staphylococcus aureus (VRSA) Real Time PCR Kit *CE Marked
11 de septiembre de 2015
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Staphylococcus aureus Real Time PCR Kit *CE Marked


DD-0041

Categorías: , , , , , , .
   
Descripción

1. Intended Use
Staphylococcus aureus real time PCR kit is used for the detection of Staphylococcus aureus in stool,
sputum, C.S.F., urine, gargle, food or water samples by the real time PCR systems.

2. Product Descriptionn
Staphylococcus aureus is a major human pathogen that causes both nosocomial and
community-acquired infections worldwide. Expression of most virulence factors is controlled by the
accessory gene regulator (agr) locus. The agr locus regulates virulence factors such as Staphylococcal
enterotoxins, toxic shock syndrome toxin-1 (tst), -toxin, -hemolysin and etc.
Staphylococcus aureus real time PCR kit contains a specific ready-to-use system for the detection of
Staphylococcus aureus by polymerase chain reaction (PCR) in the real-time PCR system. The master
contains reagents and enzymes for the specific amplification of Staphylococcus aureus DNA.
Fluorescence is emitted and measured by the real time systems´ optical unit during PCR. The detection
of amplified Staphylococcus aureus DNA fragment is performed in fluorimeter channel FAM with the
fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and sputum sample are used
for DNA extraction. In addition, the kit contains a system to identify possible PCR inhibition by
measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control
(1×107copies/ml) is supplied allows the determination of the gene load. For further information, please
refer to section 9.3 Quantitation.

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