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Streptococcus Pneumoniae Real Time PCR Kit


RD-0146

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Descripción

1. Intended Use
By using real time PCR systems, Streptococcus pneumoniae real time PCR kit is used for the detection
of Streptococcus pneumoniae in samples like nasal and pharyngeal secretions, sputum, pleural sffusion,
bronchial lavage, lung biopsy and etc.

2. Product Description
Streptococcus pneumoniae, or pneumococcus, is gram-positive, alpha-hemolytic, bile soluble
aerotolerant anaerobe and a member of the genus Streptococcus.A significant human pathogenic
bacterium, S. pneumoniae was recognized as a major cause of pneumonia in the late 19th century and
is the subject of many humoral immunity studies.The organism causes many types of pneumococcal
infection other than pneumonia, including acute sinusitis, otitis media, meningitis, bacteremia, sepsis,
osteomyelitis, septic arthritis, endocarditis, peritonitis, pericarditis, cellulitis, and brain abscess.It is the
most common cause of bacterial meningitis in adults and children.
Streptococcus pneumoniae real time PCR Kit contains a specific ready-to-use system for the detection
of the Streptococcus pneumoniae by polymerase chain reaction (PCR) in the real-time PCR system.
The master contains reagents and enzymes for the specific amplification of the Streptococcus
pneumoniae DNA. Fluorescence is emitted and measured by the real time systems´ optical unit during
the PCR. The detection of amplified Streptococcus pneumoniae DNA fragment is performed in
fluorimeter channel FAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in
the kit. An external positive control (1×107copies/ml) allows the determination of the gene load. For
further information, please refer to section 9.2 Quantitation.

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