1. Intended Use
The TTV real time PCR kit is used for the detection of TTV in serum or plasma by using real time PCR
High sensitivity: lower detection line 103 Copies/ml
High specificity: test result will be positive, only to transfusion transmitted virus.
Short operating time: 1 and a half hours totally
Good stability: kept for 12 months at –20℃; CV≤5%;
2. Product Description
The TTV real time PCR kit contains a specific ready-to-use system for TTV detection through
polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and
enzymes for the specific TTV DNA amplification. Fluorescence is emitted and measured by the real
time systems´ optical unit during PCR. The detection of amplified TTV DNA fragment is performed in
fluorimeter channel FAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in
the kit. In addition, the kit contains a system to identify possible PCR inhibition by measuring the
HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as
1×107copies/ml, is supplied which allows the determination of the gene load. For further information,
please refer to section 9.3 Quantitation.