Vibrio cholerae
11 de septiembre de 2015
Vibrio Cholerae O1 Real Time PCR Kit *CE Marked
11 de septiembre de 2015
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Vibrio Cholerae (Gene CTX) Real Time PCR Kit *CE Marked


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1. Intended Use
Vibrio cholera (Gene CTX)real time PCR kit is used for the detection of Cholera Vivrion (Gene CTX)
in stool or water samples by using real time PCR systems.

2. Product Description
Cholera is an acute intestinal infection caused by ingestion of food or water contaminated with the
bacterium Vibrio cholerae. It has a short incubation period and produces an enterotoxin that causes a
copious, painless, watery diarrhoea that can quickly lead to severe dehydration and death if treatment
is not promptly given. Vomiting also occurs in most patients.
Most persons infected with V. cholerae do not become ill, although the bacterium is present in their
faeces for 7-14 days. When illness does occur, about 80-90% of episodes are of mild or moderate
severity and are difficult to distinguish clinically from other types of acute diarrhoea. Less than 20% of
ill persons develop typical cholera with signs of moderate or severe dehydration.
Cholera remains a global threat and is one of the key indicators of social development. While the
disease no longer poses a threat to countries with minimum standards of hygiene, it remains a
challenge to countries where access to safe drinking water and adequate sanitation cannot be
guaranteed. Almost every developing country faces cholera outbreaks or the threat of a cholera
Vibrio cholera (Gene CTX)real time PCR kit contains a specific ready-to-use system for the detection
of the Vibrio cholera (Gene CTX)using PCR (polymerase chain reaction) in the real-time PCR system.
The master contains reagents and enzyme for the specific amplification of the Vibrio cholera (Gene
CTX)DNA. Fluorescence is emitted and measured by the real time systems´ optical unit during the
PCR. The detection of amplified gene CTX of Vibrio cholera DNA fragment is performed in
fluorimeter channel FAM with the fluorescent quencher BHQ1. In addition, the kit contains a system
to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal
control (IC). An external positive control defined as 1×108copies/ml is supplied which allow the
determination of the gene load. For further information, please refer to section 9.3 Quantitation.

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