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Vibrio Parahaemolyticus Real Time PCR Kit *CE Marked


DD-0038

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Descripción

1. Intended Use
Vibrio parahaemolyticus real time PCR kit is used for the detection of Vibrio parahaemolyticus in excreta or water samples by using real time PCR systems.

2.  Product Description
Vibrio parahaemolyticus real time PCR Kit contains a specific ready-to-use system for the detection of the
Vibrio parahaemolyticus by polymerase chain reaction (PCR) in the real-time PCR system. The master
contains reagents and enzymes for the specific amplification of Vibrio parahaemolyticus DNA. Fluorescence
is emitted and measured by the real time systems´ optical unit during the PCR. The detection of amplified
Vibrio parahaemolyticus DNA fragment is performed in fluorimeter channel FAM with the fluorescent
quencher BHQ1. DNA extraction buffer is available in the kit and excreta samples are used for DNA
extraction. In addition, the kit contains a system to identify possible PCR inhibition by measuring the
VIC/JOE fluorescence of the internal control (IC). An external positive control (1×10
7
copies/ml) allows the
determination of the gene load. For further information, please refer to section 10.3 Quantitation

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