1. Intended Use
Yersinia Enterocoliticareal time PCR kit is used for the detection of Yersinia Enterocoliticareal in
stool, blood，food or water samples by using real time PCR systems.
2. Product Description
Yersinia enterocolitica is a species of gram-negative coccobacillus-shaped bacterium, belonging to
the family Enterobacteriaceae. Primarily a zoonotic disease (cattle, deer, pigs, and birds), animals
which recover frequently become asymptomatic carriers of the disease.
Acute Y. enterocolitica infections produce severe diarrhea in humans, along with Peyer’s patch
necrosis, chronic lymphadenopathy, and hepatic or splenic abscesses. Additional symptoms may
include entero-colitis, fever, mesenteric adenitis, erythema nodosum and acute terminal ileitis, which
may be confused with appendicitis or Crohn’s disease.
Yersinia Enterocoliticareal time PCR kit contains a specific ready-to-use system for the detection of
the Yersinia Enterocoliticausing PCR (polymerase chain reaction) in the real-time PCR system. The
master contains reagents and enzyme for the specific amplification of the Yersinia
EnterocoliticaDNA. Fluorescence is emitted and measured by the real time systems´ optical unit
during the PCR. The detection of amplified Yersinia Enterocolitica DNA fragment is performed in
fluorimeter channel FAM with the fluorescent quencher BHQ1. In addition, the kit contains a
system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the
internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow
the determination of the gene load. For further information, please refer to section 9.3 Quantitation.