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Aggregatibacter actinomycetemcomitans


Description
The ViPrimePLUS Aggregatibacter actinomycetemcomitans qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Aggregatibacter actinomycetemcomitans genomes in clinical samples (e.g. subgingival, supragingival, saliva and dental plaque).

(RUO)

Código: QM2002

Código: QM2002. Categorías: , , , , .
   
Descripción

Description
The ViPrimePLUS Aggregatibacter actinomycetemcomitans qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Aggregatibacter actinomycetemcomitans genomes in clinical samples (e.g. subgingival, supragingival, saliva and dental plaque).

Periodontitis is the chronic degeneration of the connective tissue in the gums and alveolar bones that supports the teeth. It is the cause of dental loss after the age of 45 in many humans. It develops from the pre-existing gingivitis, the inflammation of the gum due to the long settlement of dental plagues. The pathogenic activity of the facultative anaerobes in the plague contributes to the development of the periodontal disease. A. actinomycetemcomitans is the most important periodontopathogen found in the microbiota biofilm. It releases leukotoxin which destroys neutrophils, monocytes and T-lymphocytes, therefore rendering a weakened immune system in the supragingival area. This eventually leads to the development of aggressive periodontitis. There are evidences showing the systemic side effects of periodontitis such as the increased risk of diabetes and cardiovascular diseases.

ViPrimePLUS Aggregatibacter actinomycetemcomitans qPCR Kit is intended for research use only.

Kit Component

  • A. actinomycetemcomitans Primers and Probe Mix (A. actinomycetemcomitans PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit DNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material Clinical samples (subgingival, supragingival, saliva and dental plaque)
Gene Target Haemoglobin-binding protein (hbpA) gene
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

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