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Clostridium estertheticum


Description
The ViPrimePLUS Clostridium estertheticum qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Clostridium estertheticum genomes in faecal and food samples.

(RUO)

Código: QM2016

Código: QM2016. Categorías: , , , , .
   
Descripción

Description
The ViPrimePLUS Clostridium estertheticum qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Clostridium estertheticum genomes in faecal and food samples.

Clostridium spp. represents a group of anaerobic, spore-forming, Gram positive bacteria that infects the gastrointestinal tract, causing serious diarrhea, or the nervous system, causing neurological damage. Clostridium spp. has great survival mechanism because they sporulate under extreme conditions, but transform into infectious form when the environment turns favorable again. Clostridium difficile also causes colitis, an infection of the colon, due to the administration of some broad-range antibiotic. When a majority of the normal flora in the gut is removed by the broad-spectrum antibiotic, the resilient C. difficile takes the opportunity to colonize the gut and later secrete toxins, i.e. toxin A (enterotoxin) and toxin B (cytotoxin). Toxin B is found in all strains, however toxin A is only found in 70% of them. This species is closely-related to the bacterium that causes tetanus and botulism.

ViPrimePLUS Clostridium estertheticum qPCR Kit is intended for research use only.

Kit Component

  • C. estertheticum Primers and Probe Mix (C. estertheticum PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit DNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material • Faecal samples
• Food samples
Gene Target Triosephosphate isomerase (tpi) gene
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

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Reacciones

150 rxns

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