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Entamoeba histolytica


Description
The ViPrimePLUS Entamoeba histolytica qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Entamoeba histolytica genomes in clinical samples (e.g. faeces), food samples or water samples.

(RUO)

 

Código: QM1004

Código: QM1004. Categorías: , , , , .
   
Descripción

Description
The ViPrimePLUS Entamoeba histolytica qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Entamoeba histolytica genomes in clinical samples (e.g. faeces), food samples or water samples.

Entamoeba protozoa are usually found in the large intestines of animals and humans, except E. gingivalis which resides in the mouth as a commensal. One important human pathogenic species is E. histolytica, which causes amebiasis. Most cases are asymptomatic, but bowel inflammation, dysentery and rare incidences of invasive extraintestinal disease may occur. Invasive amebiasis may result in multiorgan damage, such as amebic liver abscess due to the spread of the trophozoites in the circulatory system. Transmission is via fecal-oral route, by ingesting the cysts in contaminated food or water. Amebiasis is the fourth leading cause of death due to protozoan infections worldwide, resulting in ~70,000 deaths annually, with cases reported mostly from areas with poor sanitation.

ViPrimePLUS Entamoeba histolytica qPCR Kit is intended for research use only.

Kit Component

  • E. histolytica Primers and Probe Mix (E. histolytica PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit DNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material • Clinical samples (e.g.faeces)• Food samples• Water samples
Gene Target Internal transcribed spacer 2 (ITS2)
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

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150 rxns

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