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Enterococcus faecalis


Description
The ViPrimePLUS Enterococcus faecalis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Enterococcus faecalis genomes in clinical samples (e.g. blood, faeces and urine) and environmental samples such as water and hospital equipment.

(RUO)

Código: QM2023

Código: QM2023. Categorías: , , , , .
   
Descripción

Description
The ViPrimePLUS Enterococcus faecalis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Enterococcus faecalis genomes in clinical samples (e.g. blood, faeces and urine) and environmental samples such as water and hospital equipment.

Enterococcus sp. is a group of Gram positive bacteria which is normally found as a normal flora in human and animal gut. However, infection may occur due to the disruption of the microbial balance in the gastrointestinal tract. There are 17 species that cause clinical infections in human, especially in hospital settings. E. faecalis and E. faecium are the most prevalent species. The latter represents most of the vancomycin-resistant enterococci (VRE) strains. Others such as E. avium, E. gallinarum, E. casseliflavus, E. durans, E. raffinosus and E. mundtii are clinically significant, too. Intrinsically, most enterococci are resistant to beta-lactam antibiotics aside from their capability in acquiring resistance to a broad range of antibiotics. New antibiotics developed to counter the VRE infections may have improved the situation, however resistant strains has been reported recently. Thus, enterococci are considered the most feared nosocomial pathogens.

ViPrimePLUS Enterococcus faecalis qPCR Kit is intended for research use only.

Kit Component

  • E. faecalis Primers and Probe Mix (E. faecalis PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit DNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material • Clinical samples (e.g. blood, faeces, urine)
• Environmental samples (e.g. water, hospital equipments)
Gene Target groES heat shock protein encoding gene
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

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150 rxns

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