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Human T-lymphotropic Virus 1


Description
The ViPrimePLUS Human T-lymphotropic Virus 1 RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Human T-lymphotropic Virus 1 genome in clinical samples (e.g. blood).

(RUO)

Código: QM5057

Código: QM5057. Categorías: , , , , .
   
Descripción

Description
The ViPrimePLUS Human T-lymphotropic Virus 1 RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Human T-lymphotropic Virus 1 genome in clinical samples (e.g. blood).

Human T-Lymphotropic virus (HTLV) is a RNA retrovirus that predominantly affects the T lymphocytes in human. The Human Immunodeficiency virus (HIV) was once categorized in the HTLV genus before the reclassification in 1983. After 30 years from the initial discovery, 4 types of HTLV have been identified and among them, the most significant two are the HTLV 1 and 2, which have affected about 15-20million people worldwide. HTLV-1 is divided into 6 subclasses which are endemic to different regions. It causes multiple disorders such as the often fatal leukaemia, debilitative myelopathy, uveitis and dermatitis. However, HTLV-2, which consists of 4 subtypes, is associated with milder neurological disorder and chronic pulmonary infections. The novel HTLV-3 and HTLV-4 have been isolated in a few cases but they have little clinical significance. Mode of transmission is via breast milk, sexual contact and intravenous drug abuse.

ViPrimePLUS Human T-lymphotropic Virus 1 RT-qPCR Kit is intended for research use only.

Kit Component

  • HTLV1 Primers and Probe Mix (HTLV1 PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit RNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material Clinical samples (e.g. blood)
Gene Target Polymerase gene (Pol)
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

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