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Novel Coronavirus hCoV-EMC / MERS


Description
The ViPrimePLUS Novel Coronavirus hCOV-EMC / MERS RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of novel coronavirus (hCoV-EMC / MERS) genome in clinical samples (e.g. throat swab, nasopharyngeal aspirates and stool).

(RUO)

Código: QM5064

Código: QM5064. Categorías: , , , , .
   
Descripción

Description
The ViPrimePLUS Novel Coronavirus hCOV-EMC / MERS RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of novel coronavirus (hCoV-EMC / MERS) genome in clinical samples (e.g. throat swab, nasopharyngeal aspirates and stool).

Coronavirus family represents a large collection of viruses. These single-stranded RNA viruses are divided into groups and subgroups based on their genome sequence. Recently, a novel human coronavirus 2012 has emerged in the Middle East region. This new coronavirus is a beta-coronavirus with genetic sequence similar to bat coronaviruses.

ViPrimePLUS Novel Coronavirus hCOV-EMC / MERS RT-qPCR Kit is intended for research use only.

Kit Component

  • ORF1ab Primers and Probe Mix (ORF1ab PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit RNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material Clinical samples (e.g. throat swab, nasopharyngeal aspirates, stool)
Gene Target Open Reading Frame 1 ab and the upE region of Open Reading Frame 5
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

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