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Plasmodium falciparum


Description
The ViPrimePLUS Plasmodium falciparum qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Plasmodium falciparum genomes in clinical samples (e.g. blood).

(RUO)

Código: QM4011

Código: QM4011. Categorías: , , , , , .
   
Descripción

Description
The ViPrimePLUS Plasmodium falciparum qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Plasmodium falciparum genomes in clinical samples (e.g. blood).

Plasmodium spp. is an obligate protozoan that causes malaria, a common traveler’s disease. There are about 200 species identified, and 5 of them infect human, i.e. P. falciparum, P. malariae, P. ovale, P. knowlesi and P. vivax. These parasites exploited mosquitoes of the Aedes, Culex, Mansonia, Theobaldia and Anopheles genera to transmit them to different vertebrate host via biting. Symptoms of the infection includes fever, chills, fatigue, headache, nausea, anemia, diarrhea and in severe cases, pulmonary and renal dysfunction. Effective treatment is given based on the parasites’ species identification. Now, the gold standard of the diagnosis is microscopic examination of blood smears, which lacks of sensitivity and requires considerable expertise. Therefore, real time PCR offers a fast, sensitive and specific solution to the diagnosis.

ViPrimePLUS Plasmodium falciparum qPCR Kit is intended for research use only.

Kit Component

  • P. falciparum Primers and Probe Mix (P. falciparum PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit DNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material Clinical samples (e.g. blood)
Gene Target Plasmepsin 4 gene
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

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