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Porphyromonas gingivalis


Description
The ViPrimePLUS Porphyromonas gingivalis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of the Porphyromonas gingivalis genomes in clinical samples.

(RUO)

Código: QM2047

Código: QM2047. Categorías: , , , , .
   
Descripción

Description
The ViPrimePLUS Porphyromonas gingivalis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of the Porphyromonas gingivalis genomes in clinical samples.

Porphyromonas gingivalis is an anaerobic, rod-shaped, non-motile, Gram-negative bacterium. Infection with this bacterium can lead to gingivitis and periodontitis. The bacterium resides in the mouth within the gingival and is known to cause inflammation of this soft tissue. P. gingivalis adheres to the gum layer using the fimbriae and once attached to the gingiva, the bacterium interacts with other bacteria present here and in doing so, form a biofilm known as plaque. If left untreated, the plaque can lead to the formation of lesions between the tooth and the gingival layer. The bacterium is also able to express proteolytic enzymes, which attack the ligaments that hold the tooth and gum together. The continuation of such process eventually results in the loosening of the tooth from the gum and bone, and potentially resulting in the loss of the tooth. Symptoms caused by the infection with P. gingivalis include inflammation of gums that cause redness and may be painful, and bad breath.

ViPrimePLUS Porphyromonas gingivalis qPCR Kit is intended for research use only.

Kit Component

  • P. gingivalis Primers and Probe Mix (P. gingivalis PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit DNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material Clinical samples
Gene Target Fimbrillin fimA(I) gene
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

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