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Toxoplasma gondii


Description
The ViPrimePLUS Toxoplasma gondii qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Toxoplasma gondii genomes in clinical samples (e.g. blood, CSF, amniotic fluid), food samples or water samples.

(RUO)

Código: QM4016

Código: QM4016. Categorías: , , , , .
   
Descripción

Description
The ViPrimePLUS Toxoplasma gondii qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Toxoplasma gondii genomes in clinical samples (e.g. blood, CSF, amniotic fluid), food samples or water samples.

Toxoplasma gondii is a parasitic protozoan whose definitive host is cat. It infects many warm-blooded mammals including human; causing toxoplasmosis, a major foodborne and neonatal infectious disease. In healthy adults, the infection is usually self-limiting and normally asymptomatic although in some cases, flu-like symptoms are present. It remains in the body in a dormant stage until reactivation when the person is immunosuppressed. This patient may then develop the deadly toxoplasmic encephalitis. Due to the cross-placental ability of the parasite, women who are infected during the course of their pregnancy will pass the infection to their fetuses, bringing severe damages in the latter’s development. Among the complications are stillborn, miscarriage, babies with abnormal head size and high risk of developing ocular diseases during adulthood. Association between toxoplasma infections with schizophrenia is currently being investigated. Bearing in mind the severity of the disease, routine screening and good hygiene is of great value in helping to reduce the risk of infection.

ViPrimePLUS Toxoplasma gondii qPCR Kit is intended for research use only.

Kit Component

  • T. gondii Primers and Probe Mix (T. gondii PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit DNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material • Clinical samples (e.g. blood, CSF, amniotic fluid)
• Food samples
• Water samples
Gene Target Repeat region
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

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