1. Intended Use
Salmonella real time PCR kit is used for the detection of Salmonella in stool or water samples by
using real time PCR systems.
2. Product Description
Salmonella is a genus of bacteria that are a major cause of foodborne illness throughout the world.
The bacteria are generally transmitted to humans through consumption of contaminated food of
animal origin, mainly meat, poultry, eggs and milk. Salmonella real time PCR Kit contains a specific
ready-to-use system for the detection of the Salmonella by polymerase chain reaction (PCR) in the
real-time PCR system. The master contains reagents and enzymes for the specific amplification of the
Salmonella DNA including S.enterica, S.bongoru, S.subterranae and suspecies of S.enterica.
Fluorescence is emitted and measured by the real time systems´ optical unit during the PCR. The
detection of amplified Salmonella DNA fragment is performed in fluorimeter channel FAM with the
fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and excreta or water
samples are used for DNA extraction. In addition, the kit contains a system to identify possible PCR
inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external
positive control (1×107 copies/ml) allows the determination of the gene load. For further information,
please refer to section 9.3 Quantitation.