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Avian Influenza Virus H9 Real Time RT-PCR Kit *CE Marked


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1. Intended Use
Avian influenza virus H9 real time RT-PCR kit is used for the detection of avian influenza A
subtype H9 in human nasal and pharyngeal secretions and bird fece by using real time PCR

2. Product Description

Highly pathogenic avian influenza (HPAI) caused by certain subtypes of influenza A virus in
animal populations, particularly chickens, poses a continuing global human public health risk.
Direct human infection by an avian influenza A (H5N1) virus was first recognized during the 1997
outbreak in Hong Kong. Subsequently, human infections with avian strains of the H9 and H7
subtypes have been further documented. Avian influenza A H5 and H7 viruses can be
distinguished as “low pathogenic” and “high pathogenic” forms on the basis of genetic features of
the virus and the severity of the illness they cause in poultry; influenza H9 virus has been identified
only in a “low pathogenicity” form. Each of these three avian influenza A viruses (H5, H7, and H9)
theoretically can be partnered with any one of nine neuraminidase surface proteins; thus, there are
potentially nine different forms of each subtype (e.g., H5N1, H5N2, H5N3, H5N9).
Avian influenza virus H9 real time RT-PCR kit contains a specific ready-to-use system for the
detection of avian influenza virus H9 by Reverse Transcription Polymerase Chain Reaction
(RT-PCR) in the real-time PCR system. The master contains Super Mix for the specific
amplification of the avian influenza virus H9 RNA. The reaction is done in one step real time
RT-PCR. The first step is a reverse transcription (RT), during which the avian influenza virus H9
RNA is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the
specific gene fragments by polymerase chain reaction. Fluorescence is emitted and measured by
the real time systems´ optical unit during the PCR. The detection of amplified avian influenza virus
H9 DNA fragment is performed in fluorimeter channel HEX/VIC/JOE with the fluorescent
quencher BHQ1. In addition, the kit contains a system to identify possible PCR inhibition by
measuring the FAM fluorescence of the internal control (IC). An external positive control
(1×107 copies/ml) contained, allows the determination of the gene load. For further information,
please refer to section 9.3 Quantitation.

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