1. Purpose of the test
ADIAVET™ BoHV-4 REAL TIME kit is intended to detect the Bovine Herpesvirus type 4 (BoHV-4) using
real-time Polymerase Chain Reaction (PCR) technology from swab of bovine and from viral culture.
BoHV-4 is a DNA virus, member of the subfamily Gammaherpesvirinae. It has been isolated the first
time in 1966 in Hongary, from calves showing clinical signs of respiratory disease and
keratoconjunctivitis (Movar 33/63 strain). Since, virus isolation has been reported in Europe, North
America, Africa and Asia from animals showing a variety of clinical signs, such as conjunctivitis,
pneumonia, skin lesions, enteritis, ulcerative mammillitis, postpartum and chronic metritis. BoHV-4 can
also be isolated from healthy bovine. Therefore, it is sometimes named “passager” virus.
BoHV-4 is mainly isolated from both superior respiratory tract and genital tracks.
The multiplication of virus could take place likely in epithelial cells of mucosa.
The main site of the virus persistence seems to be the monocytes and macrophages in the organism.
The diagnosis can be performed by the detection of the virus by direct methods (viral isolation or PCR)
or by indirect immunofluorencence on cell cultures.
3. Description and purpose of the test
This test is based on enzymatic gene amplification or PCR technology.
Amplified products are detected in real-time thanks to a specific labelled hydrolysis probe (5’-
The ADIAVET™ BoHV-4 REAL TIME kit enables the simultaneous detection of:
– BoHV-4 target (probe labelled in FAM),
– GAPDH, an internal control of extraction and amplification steps specific from an
endogenous DNA (probe labelled with a fluorochrome read in the same spectra as VIC
ADIAGENE recommends using this test with DNA purification kits (Qiagen, Macherey-Nagel). Other
purification kits can be used if they have been validated by the user.