1. Purpose of the test
ADIAVET™ MYCO AV REAL TIME kit is intended to detect Mycoplasma synoviae and Mycoplasma
gallisepticum using real-time Polymerase Chain Reaction (PCR) technology from swab of poultry,
environmental specimen and bacterial culture.
2. Avian mycoplasms
Mycoplasma are known to cause economic losses in commercial poultry production. M.
gallisepticum causes chronic respiratory disease in chickens and infection sinusitis in turkeys. M.
synoviae most frequently occurs as subclinical upper respiratory infection and synovitis in chickens
Rapid identification of any of these mycoplasma organisms is of great importance to the poultry
industry. The diagnosis of avian mycoplasma infections is mainly based on culture and serology
(RSA, ELISA…) (Stipkovitch & Kempf, 1996). However culture is rather slow, tedious, expensive
and often contaminated.
Serological tests are useful but sometimes lack specificity or sensitivity (Yamamoto, 1991).
Recently PCR tests have been developed providing specific and sensitive tools for avian
mycoplasma detection (Kempf, 1997).
3. Description and purpose of the test
This test is based on enzymatic gene amplification or PCR technology.
Amplified products are detected in real-time thanks to a specific labelled hydrolysis probe (5’-
The ADIAVET™ MYCO AV REALTIME kit enables the simultaneous detection of:
– M. synoviae (probe labelled in FAM),
– M. gallisepticum (probe labelled in Cy5),
– an internal control of amplification step specific from an exogenous DNA (probe labelled
with a fluorochrome read in the same spectra as VIC and HEX).
The test detects the MG TS-11 and MS-H vaccine strains but not the MG 6/85 and MG-F vaccine
Adiagene recommends using this test with DNA purification kits (Adiagene, Qiagen and Macherey-
Nagel). Other purification kits can be used if they have been validated by the user.