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PCR Test for the Detection of M. synoviae


417999

Código: 417999. Categorías: , , .
   
Descripción

1. Purpose of the test
ADIAVET™ MS REAL TIME kit is intended to detect Mycoplasma synoviae using real-time Polymerase
Chain Reaction (PCR) technology from swab of poultry, environmental specimen and bacterial culture.
2. Mycoplasma synoviae
Mycoplasma are known to cause economic losses in commercial poultry production. M. synoviae
causes chronic respiratory disease in chickens and infection sinusitis in turkeys. Rapid identification
of any of these mycoplasma organisms is of great importance to the poultry industry. The diagnosis
of avian mycoplasma infections is mainly based on culture and serology (RSA, ELISA…) (Stipkovitch
& Kempf, 1996). However culture is rather slow, tedious, expensive and often contaminated.
Serological tests are useful but sometimes lack specificity or sensitivity (Yamamoto, 1991). Recently
PCR tests have been developed providing specific and sensitive tools for avian mycoplasma
detection (Kempf, 1997).
3. Description and purpose of the test
This test is based on enzymatic gene amplification or PCR technology.
Amplified products are detected in real-time thanks to a specific labelled hydrolysis probe (5’-
exonulease technology).
The ADIAVET™ MS REALTIME kit enables the simultaneous detection of:
– M. synoviae (probe labelled in FAM),
– an internal control of amplification step specific from an exogenous DNA (probe labelled
with a fluorochrome read in the same spectra as VIC and HEX).
The test detects the MS-H vaccine strain.
Adiagene recommends using this test with DNA purification kits (Adiagene, Qiagen and Macherey-
Nagel). Other purification kits can be used if they have been validated by the user.

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