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PCR Test for the Detection of Marek’s Disease


ADI461-100

Código: ADI461-100. Categorías: , , .
   
Descripción

1. Purpose of the test
ADIAVET™ MAREK REAL TIME kit is intended to detect the Gallid Herpesvirus 2, the Marek Virus (the
Marek desease virus) using real-time Polymerase Chain Reaction (PCR) technology from breeding air
collected in poultry farm.
2. Pathogen
Marek virus is a double strand DNA virus of the Herpesviridae family. Three serotypes of Marek virus
exist. Only the serotype 1 is pathogenic for poultry. This serotype takes together strains with different
levels of virulence among which the new emergent high virulent strain and the Rispens virus which is
used as vaccine. The strains of the serotypes 2 and 3 are non pathogens.
The oncogenic viruses are responsible of lymphoma associated to nervous and visceral tumors. Clinic
symtoms of the Marek disease can be neurological (paresis, paralysis, hanging wing…), weight loss,
anemia, lymphoma (nerve or skin damage…), morbidity rate reaching about 10%.
Two forms of Marek virus can be detected. The cell-associated form is responsible to the tumoral
processus but is non infectious. The cell-free form of the virus in sub-epidermic layer of desquamous
feather follicules is the infectious form.
Infection is spread horizontally by the air-borne route to other chickens. The virus protected inside
follicular cells shows a great resistance in the external environment.
The tumors develop slowly. Therefore, Marek disease mainly affects the animals with long economic
life (labeled hen, laying hen, reproductive hen). Chicken is the sensitive specie but turkey can be also
infected (in which the turkeys specially breed for Christmas showing long economic life).
The ubiquitous spread of Marek virus leads to vaccinate the animal with long economic life. Adapted
hygienic processes allow limiting the early contamination that occurs before the vaccine can be
efficient.
To assure an optimal efficiency of the vaccine, the Marek disease virus status of the poultry farms
should be control before the entrance of the animals.
3. Description and purpose of the test
This test is based on enzymatic gene amplification or PCR technology.
Amplified products are detected in real-time thanks to a specific labelled hydrolysis probe (5’-
exonulease technology).
The ADIAVET™ MAREK REAL TIME kit enables the simultaneous detection of:
– The Marek virus (probe labelled in FAM),
– an exogen control EPC-Ext added during the extraction that allows validating extraction
and amplification steps (probe labelled with a fluorochrome read in the same spectra as
VIC and HEX).
ADIAGENE validated the test using DNA purification kits (Qiagen). Other purification kits can be used
if they have been validated by the user.

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