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PCR Test for the Detection of Ornithobacterium rhinotracheale


416443

Código: 416443. Categorías: , , .
   
Descripción

1. Purpose of the test
ADIAVET™ ORT REAL TIME kit is intended to detect Ornithobacterium rhinotracheale using real-time
Polymerase Chain Reaction (PCR) technology from swab of chicken and turkey, as well as from
environmental specimen.
2. Pathogen
Ornithobacterium rhinotracheale is a gram negative bacillus, unique registered specie of
Ornithobacterium genus. It causes respiratory troubles by several bird species, especially by chickens
and turkeys. Clinical signs are nasal discharge, sneezing, coughing and sinusitis.
Clinical diagnosis is of poor interest because it is not specific. Serological diagnosis is made using ELISA
tests. However, these tests don’t allow diagnosing an acute infection because antibodies appear late.
Differential diagnosis (especially with Riemerella anatipestifer, Haemophilus paragallinarum,
Pasteurella multocida and Coenonia anatina) is currently achieved on bacteriological criteria.
3. Description and purpose of the test
This test is based on enzymatic gene amplification or PCR technology.
Amplified products are detected in real-time thanks to a specific labelled hydrolysis probe (5’-
exonulease technology).
The ADIAVET™ ORT REAL TIME kit enables the simultaneous detection of:
– ORT (probe labelled in FAM),
– GAPDH, an internal control of extraction and amplification steps specific from an
endogenous DNA (probe labelled with a fluorochrome read in the same spectra as VIC
and HEX).
Adiagène recommends using this test with DNA purification kits (Adiagène, Qiagen and Macherey-
Nagel). Other purification kits can be used if they have been validated by the user.

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