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PCR Test for the Detection of Q Fever and Chlamydiosis


418025

Código: 418025. Categorías: , , .
   
Descripción

1. Purpose of the test
ADIAVET™ CHLAMCOX REAL TIME kit is intended to detect Chlamydophila abortus and to detect
and/or to quantify Coxiella burnetii , using real-time Polymerase Chain Reaction (PCR) technology, from
swab, tissue and milk specimens of bovine, ovine and caprine.
2. Chlamydophila abortus and Coxiella burnetii
Chlamydophila abortus is an intracellular bacterium whose main target organ is the ruminant placenta
(bovine, caprine and ovine). This bacterium is the agent of abortion (enzootic abortive small ruminant)
and neonatal death. It also represents an emerging zoonotic risk for pregnant women. The disease can
also affect, less frequently, horses, carnivores, rabbits, swine, mousses and guinea pig. Clinical signs
induced by C. abortus infection don’t allow a clear diagnostic.
Detection of C. abortus by cell culture isolation is still regarded as the definitive test; however it is time
consuming and requires cell culture facilities. The most often achieved diagnosis is a bacterioscopic
examination of placental stamp.
The lack of sensitivity and specificity of this method has lead to the development of PCR tests.
Coxiella burnetii is a strictly intracellular Gram negative bacterium, agent of the Q fever (Burnet and
Freeman, 1937; Cox, 1938). The disease is present worldwide. C. burnetii is able to infect a large range
of hosts including humans. Q fever is a zoonose (pathology which can be transmitted to human) mainly
transmitted by ovine, bovine and caprine. Q fever usually results from inhalation of contaminated
aerosols originating mostly from dropping and body fluids of infected animals.
C. burnetii is present in reproductive apparatus, placenta and fluids produced during parturition or
abortion. The bacterium is excreted in milk and faeces of infected animals presenting no clinical signs.
Most infections are asymptomatic or subclinical by Human, but can be manifested as a flu-like disease,
or as hepatitis. A neurological involvement is also possible. Pericarditis and myocarditis are rare. C.
burnetii can induce abortions, causing economic losses in cattle. Prevention mostly consists in
precautions with pregnant females and with fluids from parturition.
Detection of C. burnetii by culture is long and difficult. Detection of antibodies doesn’t give
information on sanitary status of the animal.
The real-time PCR ADIAVET™ COX REALTIME allows the identification of the bacteria C. burnetii . It is
applicable to a large number of samples and various biological matrixes.
3. Description and purpose of the test
This test is based on enzymatic gene amplification or PCR technology.
Amplified products are detected in real-time thanks to a specific labelled hydrolysis probe (5’-
exonulease technology).
The ADIAVET™ CHLAMCOX REAL TIME kit enables the simultaneous detection of:
– Chlamydophila abortus (probe labelled in FAM),
– Coxiella burnetii (probe labelled in VIC),
– GAPDH, an internal control of extraction and amplification steps specific from an
endogenous DNA (probe labelled in Cy5).
Adiagene recommends using this test with DNA purification kits (Qiagen and Macherey-Nagel). Other
purification kits can be used if they have been validated by the user.

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