Real time PCR for detection Toxoplasma gondii.
1. Purpose of the test
ADIAVET™ TOXO REAL TIME kit is intended to detect Toxoplasma gondii parasite using real-time
Polymerase Chain Reaction (PCR) technology from swab, tissue and brain specimens of bovine, ovine
and caprine and from cat faeces.
2. Toxoplasma gondii
Toxoplasma gondii is a widespread protozoan parasite that can determine serious disease in human,
mammals and birds. Toxoplasma gondii may cause foetal resorption, abortion at any stage of
pregnancy, foetal mummification and stillbirth. In non-pregnant animals, majority of infections are
asymptomatic or of little consequence.
T.gondii is a cyst-forming coccidian parasite. Two asexual stages of development occur in the
intermediate host (cattle, horse), tachyzoite and bradyzoite, and one sexual stage (oocyst) occurs in the
definitive host (cat).
Routine diagnosis of T. gondii abortion is made by observation of clinical signs (abortion), by histology
of foetal tissue and by indirect fluorescent antibody test (IFAT), and ELISA tests on maternal and foetal
blood. Today, PCR based methods are useful diagnostic tools commonly used for detection of the
parasite in aborted foetus.
3. Description and purpose of the test
This test is based on enzymatic gene amplification or PCR technology.
Amplified products are detected in real-time thanks to a specific labelled hydrolysis probe (5’-
The ADIAVET™ TOXO REAL TIME kit enables the simultaneous detection of:
– Toxoplasma gondii (probe labelled in FAM),
– GAPDH, an internal control of extraction and amplification steps specific from an
endogenous DNA (probe labelled with a fluorochrome read in the same spectra as VIC
Adiagene recommends using this test with DNA purification kits (Qiagen and Macherey-Nagel). Other
purification kits can be used if they have been validated by the user.